Comparative efficacy of fungicide and gamma radiation for controlling blast of wheat

Maksuda Akter, Md. Abul Kashem, Humayara Zannat Swarna, Maher Afrose Nupu, Saji Moni Akter, Md. Rasel Parvez, Saji Moni Akter, Mohammad Delwar Hossain

Abstract


This research is conducted for comparison of an eco-friendly gamma radiation method and chemical fungicidal spray in reducing wheat blast incidence and severity in BINA under both artificial and natural conditions during 2019-2022. Wheat blast infection occurred on the leaves, spikes and newly emerged heads contained typical eye shaped blast symptoms. Magnaporthe oryzae Triticum (MoT), a causal agent of wheat blast was isolated by tissue panting method, cultured on OAT medium at 30 ±10C, confirmed by PCR amplified with universal MoT3 primer pairs that resulted 361 bp. The MoT inoculated (@ 5x106 CFU/mL) wheat plants successfully developed blast symptoms after 16 days in four tested wheat varieties/germplasm. An in vitro evaluation of five chemical fungicides was done against MoT where Nativo and Filia completely inhibited (100%) its radial mycelial growth. A total three spray, combination of half doses of Nativo + Filia [(0.3g + 1mL)/L] at 10 days intervals starting from one week of pre-heading or critical stage significantly reduced wheat blast incidence and severity in both artificially inoculated and natural conditions. Gamma radiated M3 population of wheat vars. also showed better performance in reduction of blast severity. Among the four doses of gamma radiation, 300 Gy significantly showed the better performance in reducing blast disease incidence and severity on wheat var. BG 26. Although combined fungicidal spray treatment (TF) significantly reduced 93.1% DI and 94.3 % DS in var. BG26 over control, but the highest percent reduction of wheat blast incidence (97.7%) and severity (98.4%) was observed on gamma irradiated (300 Gy) M3 line (BG26M3L4) under natural condition indicating gamma radiation brought a change in the genetic level positive for developing source of resistance against wheat blast. Moreover, gamma irradiated M3 line BG26M3L4 increased higher vegetative traits / yield than the combined fungicidal spray indicating gamma radiation might be a good replacement of chemical fungicides for management of blast of wheat.

Keywords


Key words: Wheat blast, fungicide, gamma irradiation, comparison, control.

Full Text:

PDF

References


REFERENCES: Bhuiyan, M., M. Malek, R. Emon, M. Khatun, M. M. Khandaker and M. Alam, 2022. Increased yield performance of mutation induced soybean genotypes at varied agro-ecological conditions. Brazilian journal of biology, 84; 255235.

Callaway, E., 2016. Devastating wheat fungus appears in asia for first time. Nature, 532(7600): 421-422.

Chowdhury, A. K., M. S. Saharan, R. Aggrawal, P. K. Malaker, N. Barma, T. Tiwari, E. Duveiller, P. K. Singh, A. K. Srivastava and K. Sonder, 2017. Occurrence of wheat blast in Bangladesh and its implications for south Asian wheat production. Indian journal of genetics plant breeding, 77(1): 1-9.

Debnath, B., A. Khan, M. Hossain, M. Rubayet and M. Miah, 2019. Morphological, pathological and cultural characteristics of Magnaporthe oryzae Triticum causing blast of wheat and its fungicidal control. Canadian journal of agriculture, 4(2): 218-227.

Edwards, K., C. Johnstone and C. Thompson, 1991. A simple and rapid method for the preparation of plant genomic DNA for pcr analysis. Nucleic acids research, 19(6): 1349.

Harun-Or-Rashid, M., M. B. Meah, M. I. Uddin, S. Ahmed and M. A. Kashem, 2019. Gamma radiated wheat for combating devastating blast disease (Magnaporthe oryzae Triticum) in Bangladesh. Agricultural science, 1(1): p1-p1.

Ikram, N., S. Dawar, Z. Abbas and M. J. Zaki, 2010. Effect of (60 cobalt) gamma rays on growth and root rot diseases in mungbean (Vigna radiata l.). Pakistan journal of botany, 42(3): 2165-2170.

Kabir, M., F. Tisha, H. Nayan, M. Islam, M. Kashem, M. Uddin, M. Islam and M. Meah, 2021. Determining an effective and economic fungicide spray schedule for reducing blast of wheat. International journal of agricultural research, innovation technology, 11(1): 10-16.

Katiyar, P., N. Pandey and S. Keshavkant, 2022. Gamma radiation: A potential tool for abiotic stress mitigation and management of agroecosystem. Plant stress: 100089.

Kohli, M., Y. Mehta, E. Guzman, L. De Viedma and L. Cubilla, 2011. Pyricularia blast-a threat to wheat cultivation. Czech journal of genetics plant breeding, 47(Special Issue): S00-04.

Maciel, J. L. N., A. L. D. Danelli, C. Boaretto and C. A. Forcelini, 2013. Diagrammatic scale for the assessment of blast on wheat spikes. Summa phytopathologica, 39: 162-166.

Malek, M., H. Begum, M. Begum, M. Sattar, M. Ismail and M. Rafii, 2012. Development of two high yielding mutant varieties of mustard ['Brassica juncea'(l.) czern.] through gamma rays irradiation. Australian Journal of crop science, 6(5): 922-927.

Mushtaq, S., M. Shafiq, M. Abid, M. A. Rana, S. Yaqub and M. S. Haider, 2019. A review on wheat streak mosaic virus (wsmv) disease complex. World journal of biology biotechnology, 4(1): 29-33.

Nazir, M., 2021. Salinity stress resistance in wheat. World journal of biology biotechnology, 6(2): 27-31.

Peng, J., Y. Zhou and Z. He, 2011. Global warning against the spread of wheat blast. Journal of triticeae crops 31(5): 989-993.

Pieck, M. L., A. Ruck, M. L. Farman, G. L. Peterson, J. P. Stack, B. Valent and K. F. Pedley, 2017. Genomics-based marker discovery and diagnostic assay development for wheat blast. Plant Disease, 101(1): 103-109.

Sarker, S., M. Rahman, M. Islam, S. Hasna and M. Islam, 2014. Effect of gamma radiation on morpho-physiological characters of soybean. Journal of environmental science natural resources, 7(2): 25-30.

Singh, D., 2017. Wheat blast—a new challenge to wheat production in South Asia. Indian phytopathology, 70(2): 169-177.

Wedajo, B. J. V. M., 2015. Compatibility studies of fungicides with combination of trichoderma species under in vitro conditions. 4(2): 149-153.

Zadoks, J. C., T. T. Chang and C. F. Konzak, 1974. A decimal code for the growth stages of cereals. Weed research, 14(6): 415-421.




DOI: https://doi.org/10.33865/wjb.007.03.0599

Refbacks

  • There are currently no refbacks.




Copyright (c) 2022 Maksuda Akter, Md. Abul Kashem, Humayara Zannat Swarna, Maher Afrose Nupu, Saji Moni Akter, Md. Rasel Parvez, Mohammad Delwar Hossain

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Print ISSN: 2522-6746 : Online ISSN: 2522-6754
1. How to register 
2. How to reset password
2. How to prepare a manuscript before submission 
3. How to submit a paper 
4. How to check the review status of a paper
5. How to check the plagirisim or similarity report