Morpho-molecular identification of Fusarium oxysporum as the causal agent of dry rot of carrot in Pakistan

Salman Ghuffar, Muhammad Sajjad Saeed, Waseem Abbas, Muhammad Iqbal, Nasir Mehmood, Umar Sabtain, Muhammad Ussama Yasin, Abdul Qayyum, Muhammad Rauf

Abstract


Fusarium  rot or dry rot  is an emerging threat to carrot field, which is responsible for significant economic losses worldwide. The objective of this study was to identify Fusarium species associated with  dry rot of carrot (Daucus carota).For this porpuse, ten isolates of Fusarium sp. were collected from symptomatic carrot roots at Vegetable Research Station Sahiwal (VRSS). During morphological studies, fungal colonies were observed whitish, cottony with abundunt arial mycelium. In microscopic observations, macroconidia was falciform and measured 12.1 to 30.7 (L) × 3.6 to 5.8 (W) μm with 2 to 3 septations, while elliptic microconidia of 5.8 to 8.6 (L) × 2.9 to 3.6 (W) μm with none or one septate. For molecular characterization, three isolates (ON955520, ON955053 & ON955054) were amplified using ITS1 & ITS4 primers. Sequence comparison revealed 99-100% genetic homology with previously reported isolates of F.oxysporum. To our knowledge, the occurrence of fusarium rot caused by F.oxysporum on carrot field is the first time reported in Pakistan which can cause reduction in crop yield.

Keywords


Keywords: Morphological characterization, molecular identification, ITS primer, sequencing, phylogenetic analysis.

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References


REFERENCES: Abbas, M. F., 2019. Prevalence and characterization of fungal pathogens associated with loquat (Eriobotrya japonica) in Punjab Pakistan. PMAS-Arid Agriculture University, Rawalpindi.

Achari, S. R., J. Kaur, Q. Dinh, R. Mann, T. Sawbridge, B. A. Summerell and J. Edwards, 2020. Phylogenetic relationship between australian Fusarium oxysporum isolates and resolving the species complex using the multispecies coalescent model. BMC genomics, 21(1): 1-20.

Agrawal, S., K. Singh and S. J. A. Lal, 1993. Plant protection of lentil in India. pp: 147-165.

Aoki, T., K. O'Donnell, Y. Homma and A. R. Lattanzi, 2003. Sudden-death syndrome of soybean is caused by two morphologically and phylogenetically distinct species within the Fusarium solani species complex—f. Virguliforme in North America and F. Tucumaniae in South America. Mycologia, 95(4): 660-684.

Burgess, L., P. E. Nelson and B. J. J. F. L. Summerell, 1989. Variability and stability of morphological characters of Fusarium oxysporum isolated from soils in Australia. Mycologia, 81(5): 818-822.

Chandra, N. S., E. Wulff, A. Udayashankar, B. Nandini, S. Niranjana, C. Mortensen and H. Prakash, 2011. Prospects of molecular markers in Fusarium species diversity. Applied microbiology biotechnology, 90: 1625-1639.

Chaudhary, R. and A. Kaur, 2002. Wilt disease as a cause of shift from lentil cultivation in Sangod Tehsil of Kota (Rajasthan). Indian journal of pulses research, 15(2): 193-194.

Fravel, D., C. Olivain and C. Alabouvette, 2003. Fusarium oxysporum and its biocontrol. New phytologist, 157(3): 493-502.

Geiser, D. M., 2004. Practical molecular taxonomy of fungi. Advances in fungal biotechnology for industry, agriculture, medicine: 3-14.

Ghuffar, S., M. Z. Ahmad, G. Irshad, M. A. Zeshan, A. Qadir, H. A. Anwaar, M. Z. Mansha, H. M. Asadullah, A. Abdullah and U. Farooq, 2021. First report of Aspergillus niger causing black rot of grapes in Pakistan. Plant disease, 105(05): 1570.

Gullino, M. L., J. Katan, A. J. F. w. o. g. v. Garibaldi and o. crops, 2012. The genus fusarium and the species that affect greenhouse vegetables and ornamentals. American phytopathical society: pp: 5-9.

Hall, T. A., 1999. Bioedit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/nt. In: Nucleic acids symposium series. [London]: Information retrieval Ltd., c1979-c2000.: pp: 95-98.

Hussain, A., M. Arif and A. Abbas, 2017. Management of aphid borne potato virus y (PVY) through chemical and bio-chemical methods. World journal of biology and biotechnology, 2(2): 159-163.

Khoo, H.-E., K. N. Prasad, K.-W. Kong, Y. Jiang and A. Ismail, 2011. Carotenoids and their isomers: Color pigments in fruits and vegetables. Molecules, 16(2): 1710-1738.

Kumar, S., G. Stecher and K. Tamura, 2016. Mega7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular biology evolution, 33(7): 1870-1874.

Leslie, J., B. J. J. F. L. Summerell and E. Brett A. Summerell., 2006. The Fusarium laboratory manual blackwell. 1-388.

Mandhare, V., G. Deshmukh, J. Patil, A. Kale and R. MPKV, 2011. Morphological, pathogenic and molecular characterization of Fusarium oxysporum f. Sp. Ciceri isolates from maharashtra, India.

Milićević, D. R., M. Škrinjar and T. Baltić, 2010. Real and perceived risks for mycotoxin contamination in foods and feeds: Challenges for food safety control. Toxins, 2(4): 572-592.

Mule, G., M. Gonzalez-Jaen, L. Hornok, P. Nicholson and C. Waalwijk, 2005. Advances in molecular diagnosis of toxigenic Fusarium species: A review. Food additives contaminants, 22(4): 316-323.

Nelson, P. E., T. A. Toussoun and W. Marasas, 1983. Fusarium species: An illustrated manual for identification.

Noor, A., K. Ziaf, M. A. Ghani, C. M. Ayub, I. Ahmad and M. J. P. Amjad, 2020. 35. Plant spacing effects on seed yield and quality of carrot cultivar t-29. Pure and applied biology, 9(4): 2563-2570.

Que, F., X.-L. Hou, G.-L. Wang, Z.-S. Xu, G.-F. Tan, T. Li, Y.-H. Wang, A. Khadr and A.-S. Xiong, 2019. Advances in research on the carrot, an important root vegetable in the apiaceae family. Horticulture research, 6.

Seifert, K. A., 2001. Fusarium and anamorph generic concepts. In: Fusarium: Paul E. Nelson memorial symposium amerocam phytopathological society, St. Paul, MN.

Stoilova, T. and P. Chavdarov, 2006. Evaluation of lentil germplasm for disease resistance to Fusarium wilt (Fusarium oxysporum f. Sp. Lentis). Journal of central european agriculture, 7(1): 121-126.

Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin and D. G. Higgins, 1997. The clustal_x windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic acids research, 25(24): 4876-4882.

Urooj, M., U. Arif and A. Intikhab, 2016. A brief review for identification and detection of potato viruses. World journal of biology and biotechnology, 1(1): 33-37.

Visser, M., 2006. Molecular biological studies of the fusarium wilt pathogen of banana in south africa. University of Pretoria.

Waalwijk, C., R. van der Heide, I. de Vries, T. van der Lee, C. Schoen, G. Costrel-de Corainville, I. Häuser-Hahn, P. Kastelein, J. Köhl and P. Lonnet, 2004. Quantitative detection of Fusarium species in wheat using taqman. European journal of plant pathology, 110: 481-494.

Zhang, X., J. Hu, H. Zhou, J. Hao, Y. Xue, H. Chen and B. Wang, 2014. First report of Fusarium oxysporum and F. Solani causing Fusarium dry rot of carrot in China. Plant disease, 98(9): 1273-1273.




DOI: https://doi.org/10.33865/wjb.008.01.0730

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Copyright (c) 2023 Salman Ghuffar, Muhammad Sajjad Saeed, Waseem Abbas, Muhammad Iqbal, Nasir Mehmood, Umar Sabtain, Muhammad Ussama Yasin, Abdul Qayyum, Muhammad Rauf

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