Morpho-molecular identification of alkaline protease producing bacteria from municipal solid wastes in Bangladesh

Abstract

Alkaline proteases have become important enzymes that find widespread use in diverse industrial applications. This research was aimed to isolate alkaline protease producing bacterial isolates from protein-based waste. In the current study, proteolytic bacteria were isolated and screened from protein-based wastes. Depending upon the maximum relative diameter of the clear zones, 3 isolates were selected for identification by culturing at alkaline (pH 9) media. The selected isolates were identified as G+ve Firmicutes (isolate 1) and G-ve proteobacteria (isolate 2 & 3) based on cultural, morphological and biochemical characteristics. The 16S rDNA was amplified through polymerase chain reaction followed by sequencing. Due to insufficient sequence length, first isolate has not been identified at molecular level. Isolate 2 and 3 have been identified as Stenotrophomonas maltophilia and Pseudomonas hibiscicola by using different bioinformatics tools. Our study revealed that the bacterial extracellular alkaline protease can be produced from selected bacterial strains which might be potential for industrial and biotechnological applications.