Characterization and in-vivo toxicological evaluation of imidacloprid nanoemulsion in rats

Helmi M. El-bendary, Abdel moniem S. Hassan, Amr R. Zaki, Fatma I. Abo El-Ela

Abstract


Acute pesticide poisoning is an important public health problem worldwide and accounts for a significant number of deaths occurring each year. The present article aimed to investigate toxic effects of imidacloprid (IMD) nanoemulsion formulated using ultrasound dispersion technique, and characterized using FTIR, TEM anddynamic light scattering in adult rats. The synthesized Nano-emulsion droplets are mainly spherical in shape and their sizes ranged between (19nm –128 nm) with zeta potential of −38.8± 0.mV. Also, The median lethal dose (LD50) of nano imidacloprid in rats was 39 mg/kg body weight. Administration of different doses of 3, 1.4 and 0.8 mg.kg mg/kg b.wt. of IMD Nano emulsion to rats for 21 days, adversely affects the body weight and weight gain, and resulted in a significant increase in serum serum ALT, AST activities , glucose, Creatinine, urea and cholesterol concentrations, as well as reduced serum total proteins, Albumin and globulin as compared to control rats. The results clearly suggest that treatment with IMD nanoemulsion adversely affects the liver & kidney functions which confirmed by the histopathological findings. Nanoemulsion form and also increases the DNA damage as confirmed by the comet test

Keywords


Imidacloprid, nanoemulsion, toxicity, comet assay, rats

Full Text:

PDF

References


Balani, T., S. Agrawal and A. Thaker, 2011. Hematological and biochemical changes due to short-term oral administration of imidacloprid. Toxicology international, 18(1): 2.

Buege, J. and S. Aust, 1978. Microsomal lipid peroxidation methods,. Enzymol. pp: 302–310.

Fatma I. Abo El-Ela, S. A. S. Shany, B. Manal, El-Deen,, , H. A. El-Banna, A. A. El-Gendy, K. Hendy and M. A. Tohamy, 2016. Investigating the potential role of vitamin e in modulating the immunosuppressive effects of tylvalosin and florfenicol in broiler chickens. Research in veterinary science, 108: 25-32.

Garrido-Herrera, F., E. Gonzalez-Pradas and M. Fernández-Pérez, 2006. Controlled release of isoproturon, imidacloprid, and cyromazine from alginate− bentonite-activated carbon formulations. Journal of agricultural food chemistry, 54(26): 10053-10060.

Lonare, M., M. Kumar, S. Raut, P. Badgujar, S. Doltade and A. Telang, 2014. Evaluation of imidacloprid-induced neurotoxicity in male rats: A protective effect of curcumin. Neurochemistry international, 78: 122-129.

Memarizadeh, N., M. Ghadamyari, M. Adeli and K. Talebi, 2014. Preparation, characterization and efficiency of nanoencapsulated imidacloprid under laboratory conditions. Ecotoxicology environmental safety, 107: 77-83.

Miyamae, Y., K. Iwasaki, N. Kinae, S. Tsuda, M. Murakami, M. Tanaka and Y. F. Sasaki, 1997. Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (comet) assay.: 2. Relationship between DNA migration and alkaline condition. Mutation research/genetic toxicology environmental mutagenesis, 393(1-2): 107-113.

Ncibi, S., M. B. Othman, A. Akacha, M. N. Krifi and L. Zourgui, 2008. Opuntia ficus indica extract protects against chlorpyrifos-induced damage on mice liver. Food chemical toxicology, 46(2): 797-802.

Salim, N., M. Basri, M. A. Rahman, D. Abdullah, H. Basri and A. Salleh, 2011. Phase behaviour, formation and characterization of palm-based esters nanoemulsion formulation containing ibuprofen. Nanomedic nanotechnol, 2(4): 1-5.

Schneider, C. A., W. S. Rasband and K. W. Eliceiri, 2012. Nih image to imagej: 25 years of image analysis. Natural Methods journal, 9: 671-675.

Shahavi, M. H., M. Hosseini, M. Jahanshahi, R. L. Meyer and G. N. Darzi, 2019. Evaluation of critical parameters for preparation of stable clove oil nanoemulsion. Arabian journal of chemistry, 12(8): 3225-3230.

Singare, D. S., S. Marella, K. Gowthamrajan, G. T. Kulkarni, R. Vooturi and P. S. Rao, 2010. Optimization of formulation and process variable of nanosuspension: An industrial perspective. International journal of pharmaceutics, 402(1-2): 213-220.

Vohra, P., K. S. Khera and G. K. Sangha, 2014. Physiological, biochemical and histological alterations induced by administration of imidacloprid in female albino rats. Pesticide biochemistry physiology, 110: 50-56.

Wan-Jun, S., S. Wei-Wei, G. Sai-Yan, L. Yi-Tong, C. Yong-Song and Z. Pei, 2010. Effects of nanopesticide chlorfenapyr on mice. Toxicological environment chemistry, 92(10): 1901-1907.

Yanai, T., T. Sakai, H. Fukushi, K. Hirai, M. Narita, H. Sakai and T. Masegi, 1998b. Neuropathological study of gazelle herpesvirus 1 (equine herpesvirus 9) infection in thomson's gazelles (gazella thomsoni). The journal of comparative pathology, 119: 159-168.




DOI: https://doi.org/10.33865/wjb.06.01.0422

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2021 Helmi M. El-bendary, Abdel moniem S. Hassan, Amr R. Zaki, Fatma I. Abo El-Ela

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Print ISSN: 2522-6746 : Online ISSN: 2522-6754
1. How to register 
2. How to reset password
2. How to prepare a manuscript before submission 
3. How to submit a paper 
4. How to check the review status of a paper
5. How to check the plagirisim or similarity report