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Molecular detection of Babesia bovis along with haemato-biochemical alterations in large ruminants associated with babesiosis in central Punjab, Pakistan

Mubarik Ali, Muhammad Jamil, Norina Jabeen, Jaweria Gul, Muhammad Kashif, Imtiaz Khan, Naimat Ullah


Babesiosis is endemic in Pakistan and is one of the most important bovine diseases that cause huge economic losses and high mortality in young animals. A hematobiochemical study was conducted to unveil the difference between diseased and healthy animals in specific districts i.e., Faisalabad, Toba Tek Singh and Jhang of Punjab, Pakistan. The total number of blood samples collected were 518 which include samples of 158 buffaloes and 360 cattle. The collected samples are then analyzed with the help of PCR (Polymerase Chain Reaction) targeting apocytochrome b-genes (CYTb) followed by hematobiochemical analysis. The analysis of data was done by the Chi-square test. The PCR tests conducted in summer suggests that 53 out of 180 (29.4%) cows and 19 out of 79 (24.05%) buffaloes are prone to babesiosis. On the other hand, in winter results showed that 12.7 (23/180), 13.92 % (11/79) samples positive for Babesia genus through c-PCR. The positive samples were further investigated for hematological and biochemical analysis. The results revealed that, the mean value of hematological parameters like RBCs, Hb, PCV, MCV and MCHC was significantly (P< 0.05) decreased in infected animals (cows and buffaloes) as compared to the non-infected ones. While the biochemical parameters like ALT, AST, Cholesterol and LDH were significantly (P< 0.05) increased in infected animals as compared to healthy animals. These findings are the novel molecular and hematobiochemical evidence of B. bovis in dairy herds of Punjab province, Pakistan.


Babesiosis, PCR, hematobiochemical, B. bovis, animals, infected

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Alam, T. and S. Nasr, 2011. Hematological and biochemical investigation in bovine babesiosis and theileriosis. Benha Veterinary Medical Journal, 22(2): 118-126.

Aulakh, G., L. Singla, P. Kaur and A. Alka, 2005. Bovine babesiosis due to babesia bigemina haematobiochemical and therapeutic studies. The Indian Journal of Animal Sciences, 75(6).

Aziz, P., S. Marodia, P. Ganesan and C. Sharma, 2020. A clinical study on hemato-biochemical changes in cows affected with babesiosis. Pharm. Innov. J, 9(2): 242-245.

Babes, V., 1888. Sur l’hemoglobinurie bacterienne du boeuf. CR Acad. Sci, 107: 692-694.

Durrani, A. and N. Kamal, 2008. Identification of ticks and detection of blood protozoa in friesian cattle by polmerase chain reacton test and estimation of blood parameters in district kasur, pakistan. Tropical animal health and production, 40(6): 441-447.

Esmaeilnejad, B., M. Tavassoli and S. Asri-Rezaei, 2012. Investigation of hematological and biochemical parameters in small ruminants naturally infected with babesia ovis. In: Veterinary Research Forum. Faculty of Veterinary Medicine, Urmia University, Urmia, Iran: pp: 31.

Ganguly, A., R. Bisla, I. Ganguly, H. Singh, V. Bhanot and S. Chaudhri, 2017. Direct blood pcr detection of babesia bigeminaand its effect on haematological and biochemical profile in crossbred cattle of eastern haryana. Indian J. Anim. Res, 51(1): 141-145.

Gungi, S., G. Haritha and K. Kumari, 2016. Clinical management of babesiosis in cattle: A case report. Res. J. Vet. Pract, 4(2): 30-33.

Hassanpour, A., Y. G. Sabegh, A. Sadeghi-nasab and A. Hassanpour, 2013. Assessment of serum antioxidant enzymes activity in cattle suffering from theileriosis. Eur. J. Exp. Biol, 3(1): 493-496.

Hussein, A., N. Mohammed and H. Mohammed, 2007. Theileriosis and babesiosis in cattle: Haemogram and some biochemical parameters. In: Proceedings of XIII International Congress of International Society of Animal Hygiene. pp: 21.

Ibrahim, S. O. A., 2017. Gastrointestinal helminth parasites of ruminants slaughtered in shendi abattoir, river nile state, sudan. Yasser Abdelmaged Suleiman.

Iqbal, F., M. Fatima, S. Shahnawaz, M. Naeem, R. Shaikh, M. Ali, A. Shaikh and M. Aktas, 2011. A study on the determination of risk factors associated with babesiosis and prevalence of babesia sp., by pcr amplification, in small ruminants from southern punjab (pakistan). Parasite: journal de la Société Française de Parasitologie, 18(3): 229.

Jayalakshmi, K., M. Sasikala, S. Kavitha, R. Ravi, M. Veeraselvam and S. Krishnakumar, 2017. Haematological and biochemical alterations in babesiosis of crossbred cow. Indian veterinary journal, 94(04): 81-82.

Kerr, M. G., 2008. Veterinary laboratory medicine: Clinical biochemistry and haematology. John Wiley & Sons.

Lotfollahzadeh, S., M. Rahmani, M. Mohri and O. Madadgar, 2012. Changes in serum iron concentration and hepatic enzyme activities in cattle infected with theileria annulata and babesia bigemina. Comparative Clinical Pathology, 21(5): 829-832.

M’ghirbi, Y., A. Hurtado, J. Brandika, K. Khlif, Z. Ketata and A. Bouattour, 2008. A molecular survey of theileria and babesia parasites in cattle, with a note on the distribution of ticks in tunisia. Parasitology research, 103(2): 435-442.

Mahmoud, M. S., O. M. Kandil, S. M. Nasr, S. H. Hendawy, S. M. Habeeb, D. M. Mabrouk, M. G. Silva and C. E. Suarez, 2015. Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in egypt. Parasites & Vectors, 8(1): 1-15.

Moretti, A., V. Mangili, R. Salvatori, C. Maresca, E. Scoccia, A. Torina, I. Moretta, S. Gabrielli, M. P. Tampieri and M. Pietrobelli, 2010. Prevalence and diagnosis of babesia and theileria infections in horses in italy: A preliminary study. The Veterinary Journal, 184(3): 346-350.

Mosqueda, J., A. Olvera-Ramirez, G. Aguilar-Tipacamu and G. J Canto, 2012. Current advances in detection and treatment of babesiosis. Current medicinal chemistry, 19(10): 1504-1518.

Murray, R. K., D. K. Granner, P. Mayes and V. W. Rodwell, 2000. Harper’s biochemistry, appleton and lange. Stamford, CT.[Google Scholar].

Radostits, O. M., C. Gay, K. W. Hinchcliff and P. D. Constable, 2006. Veterinary medicine e-book: A textbook of the diseases of cattle, horses, sheep, pigs and goats. Elsevier Health Sciences.

Razavi, S., S. Nazifi, E. Rakhshandehroo, P. Firoozi and M. Farsandaj, 2012. Erythrocyte antioxidant systems, lipid peroxidation and circulating lipid profiles in cattle naturally infected with theileria annulata. Rev. Med. Vet, 163(1): 18-24.

Rehman, W., I. A. Khan, A. Qureshi and S. Hussain, 2004. Prevalence of different species of ixodidae (hard ticks) in rawalpindi and islamabad. Pakistan Journal of Medical Science, 43: 42-46.

Salem, N. Y., S. G. Yehia, H. S. Farag and M. A. Elkhiat, 2016. Clinical, hemato-biochemical alterations and oxidant–antioxidant biomarkers in babesia-infected calves. International journal of veterinary science and medicine, 4(1): 17-22.

SAUD, N., F. Ahmed, I. Sheikh and M. Bhattacharya, 2005. Prevalence of bovine babesiosis in dirang valley of arunachal pradesh. Indian veterinary journal, 82(9): 1011-1012.

Shahnawaz, S., M. Ali, M. Aslam, R. Fatima, Z. Chaudhry, M. Hassan and F. Iqbal, 2011. A study on the prevalence of a tick-transmitted pathogen, theileria annulata, and hematological profile of cattle from southern punjab (pakistan). Parasitology research, 109(4): 1155-1160.

Shahzad, W., N. Haider, A. Mansur-ud-Din, R. Munir, M. S. Saghar, M. H. Mushtaq, N. Ahmad, G. Akbar and F. Mehmood, 2013. Prevalence and molecular diagnosis of babesia ovis and theileria ovis in lohi sheep at livestock experiment station (les), bahadurnagar, okara, pakistan. Iranian Journal of Parasitology, 8(4): 570.

Shaikh, R. S., K. Ramzan, S. Nazli, S. Sattar, S. N. Khan, S. Riazuddin, Z. M. Ahmed, T. B. Friedman and S. Riazuddin, 2005. A new locus for nonsyndromic deafness dfnb51 maps to chromosome 11p13-p12. American journal of medical genetics. Part A, 138(4): 392.

Sharma, A., L. D. Singla, B. K. Batth and P. Kaur, 2016. Clinicopatho-biochemical alterations associated with subclinical babesiosis in dairy animals. Journal of arthropod-borne diseases, 10(2): 258.

Talkhan, O., M. Radwan and M. Ali, 2010. Cattle babesiosis and associated biochemical alteration in kalubyia governorate. Nat Sci, 12: 24-27.

Tufani, N., M. Fazili, H. Malik, S. Beigh and K. H. Dar, 2015. Clinico haematological profile and therapeutic management of acute babesiosis in a holstein-friesian crossbred cow. Veterinary Clinical Science, 3(3): 11-14.

Ziapour, S. P., B. Esfandiari and M. R. Youssefi, 2011. Study of the prevalence of babesiosis in domesticated animals with suspected signs in mazandaran province, north of iran, during 2008. Journal of Animal and Veterinary Advances, 10(6): 712-714.

Zulfiqar, S., S. Shahnawaz, M. Ali, A. M. Bhutta, S. Iqbal, S. Hayat, S. Qadir, M. Latif, N. Kiran and A. Saeed, 2012. Detection of babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from southern punjab. Asian Pacific journal of tropical biomedicine, 2(2): 104-108.



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