Antihyperlipidemic activity of hydroalcoholic seed extract of Cucumis sativus L in triton X-100 induced hyperlipidemic rats

Tayyaba Aftab, Atiq ur Rehman, Abdullah Abdo Albegali, Amir Rashid, Ahsan Anjum, Mayada Mohammed, Hira Javed

Abstract


Hyperlipidemia is linked to an elevated risk of atherosclerosis disease and managing this risk factor is crucial. This study was aimed at investigation of high performance liquid chromatographic (HPLC) analysis and antihyperlipidemic effect of hydroalcoholic extract of seeds of the plant Cucumis sativus L in Wistar albino rats. The rats were divided into groups A, B, C and D. All the groups except group A were administered with diet having high cholesterol and given intraperitoneal injection of Triton X-100 to induce hyperlipidaemia. Group A served as normal. Group B served as hyperlipidemic control. Group C (standard) and D (plant treated) were administered with simvastatin (10 mg/kg) and hydroalcoholic extract of the plant (400 mg/kg) respectively daily for 7 days after one week of inducing hyperlipidemia. The biochemical parameters of all the groups were estimated after one week of administration of plant extract using kits of Roche Cobas C111 chemistry analyzer. Histological studies of liver and heart tissues were also done. HPLC study showed the presence of compounds in the extract. The plant decreased the levels of total cholesterol, triglycerides, alanine aminotransferase, low and very low-density lipoprotein cholesterol significantly. The plant may be used as dietary supplement in combating hyperlipidemia.

Keywords


Cucumis sativus L, HPLC, dyslipidemia, Triglycerides, Triton X-100, Simvastatin

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References


Abdelgadir, A. A., H. M. Hassan, A. M. Eltaher, G. A. Khnsaa Mohammed, A. A. Lamya Mohammed and T. B. Hago, 2020. Hypolipidemic effect of cinnamon (Cinnamomum zeylanicum) bark ethanolic extract on triton X-100 induced hyperlipidemia in albino rats. Medicinal and aromatic plants (Los Angeles), 9(351): 2167-0412.

Abou-Zaid, M. M., D. A. Lombardo, G. C. Kite, R. J. Grayer and N. C. Veitch, 2001. Acylated flavone C-glycosides from Cucumis sativus. Phytochemistry, 58: 167-172. https://doi.org/10.1016/S0031-9422(01)00156-X

Aladaileh, S.H., S.A.M. Saghir, K. Murugesu, A. Sadikun, A. Ahmad, G. Kaur, A.M. Mahmoud and V. Murugaiyah, 2019. Antihyperlipidemic and antioxidant effects of Averrhoa carambola extract in high-fat diet-fed rats. Biomedicines, 72(7).

https://doi.org/10.3390/biomedicines7030072

Berrougui, H., A. Ettaib, M. H. Gonzalez, M. A. De Sotomayor, N. Bennani-Kabchi and M. Hmamouchi, 2003. Hypolipidemic and hypocholesterolemic effect of argan oil (Argania spinosa L.) in Meriones shawi rats. Journal of ethnopharmacology, 89(1): 15-18. https://doi.org/10.1016/S0378-8741(03)00176-4

Begum, H. A., F. Asad, A. Sadiq, S. Mulk and K. Ali, 2018. Antioxidant, antimicrobial activity and phytochemical analysis of the seeds extract of Cucumis sativus Linn. Pure and applied biology, 8(1): 433-441.

http://dx.doi.org/10.19045/bspab.2018.700202

Firdous, S. M., S. Hazra, S. C. Gopinath, G. E. El-Desouky and M. A. Aboul-Soud, 2021. Antihyperlipidemic potential of diosmin in Swiss Albino mice with high-fat diet induced hyperlipidemia. Saudi journal of biological sciences, 28(1): 109-115.

https://doi.org/10.1016/j.sjbs.2020.08.040

Friedewald, W.T., R.I. Levy and D.S. Fredrickson, 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry, 18: 499-502.

https://doi.org/10.1093/clinchem/18.6.499

Fu, J., X. W. Zhang, K. Liu, Q. S. Li, L. R. Zhang, X. H. Yang and H. L. Zhu, 2012. Hypolipidemic activity in Sprague–Dawley rats and constituents of a novel natural vegetable oil from Cornus wilsoniana fruits. Journal of food science, 77(8): H160-H169.

https://doi.org/10.1111/j.1750-3841.2012.02786.x

Garaiova, I., J. Muchova, Z. Nagyova, C. Mislanova, S. Oravec, A. Dukat and Z. Durackova, 2013. Effect of a plant sterol, fish oil and B vitamin combination on cardiovascular risk factors in hypercholesterolemic children and adolescents: a pilot study. Nutrition journal, 12(1): 1-8.

Ghuffar, A., T. Ahmad and M.N. Mushtaq, 2014. Antihyperlipidemic effect of Berberis orthobotrys in hyperlipidemic animal models. Bangladesh journal of pharmacology, 9: 77–382. 10.3329/bjp.v9i3.19922

Keshetty, V., S. Pabba, R. Gudipati, J. M. Kandukuri and V. Allenki, 2009. Antihyperlipidemic activity of methanolic extract of Garlic (Allium sativum L.) in Triton X-100 induced hyperlipidemic rats. Journal of pharmacy research, 2(5): 23-31.

Kumar, D., S. Kumar, J. Singh, B. D. Vashistha and N. Singh, 2010. Free radical scavenging and analgesic activities of Cucumis sativus L. fruit extract. Journal of young pharmacists, 2(4): 365-368. https://doi.org/10.4103/0975-1483.71627

Kelishadi, R., M. Mansourian, M. Ghanadian, E. Ataei and P. Mirmoghtadaee, 2016. Nutraceuticals in hyperlipidemic children: a systematic review and meta-analysis. International Journal of pediatrics, 4: 1569-78.

Mirmiran, P., M. R. Fazeli, G. Asghari, A. Shafiee and F. Azizi, 2010. Effect of pomegranate seed oil on hyperlipidaemic subjects: a double-blind placebo-controlled clinical trial. British journal of nutrition, 104(3), 402-406.

https://doi.org/10.1017/S0007114510000504

Minaiyan, M., B. Zolfaghari and A. Kamal, 2011. Effect of hydroalcoholic and buthanolic extract of Cucumis sativus seeds on blood glucose level of normal and streptozotocin-induced diabetic rats. Iranian journal of basic medical sciences, 14(5): 436-42.

OECD, 2008. OECD guidelines for the testing of chemicals, Test 425. OECD Publishing, Paris.

Organization, W.H., 2019. WHO | Cholesterol. Global Health Observatory (GHO) data. WHO.

Rayees, B., M. Dorcus and S. Chitra, 2013. Nutritional composition and oil fatty acids of Indian winter melon Benincasa hispida (Thunb.) seeds. International food research journal, 20(3): 1151.

Ridker, P.M., N. Rifai, N.R. Cook, G. Bradwin and J.E. Buring, 2005. Non–HDL cholesterol, apolipoproteins AI and B100, standard lipid measures, lipid ratios, and CRP as risk factors for cardiovascular disease in women. The journal of American medical association, 294: 326-333. doi:10.1001/jama.294.3.326

Saeedi, R., A. Sultana and K. Rahman, 2020. Ethnomedicinal uses and pharmacological activities of different parts of Cucumis sativus Linn: an update. International journal of pharmaceutical sciences and research, 11: 1549-1556.

doi:10.13040/IJPSR.0975-8232.11(4).1549-56

Sharmin, R., M. R. I. Khan, M. A. Akhtar, A. Alim, M. A. Islam, A. S. M. Anisuzzaman and M. Ahmed, 2013. Hypoglycemic and hypolipidemic effects of cucumber, white pumpkin and ridge gourd in alloxan induced diabetic rats. Journal of scientific research, 5(1): 161-170. https://doi.org/10.3329/jsr.v5i1.10252

Shinde, S., N. Chivate, P. Kulkarni and N. Naikwade, 2013. Hypolipidemic activity of Psidium guajava Linn leaves extracts in hyperlipidemic rats. International journal of pharmacy and pharmaceutical sciences, 5: 70-72.

Soltani, R., M. Hakimi, S. Asgary, S. M. Ghanadian, M. Keshvari and N. Sarrafzadegan, 2014. Evaluation of the effects of Vaccinium arctostaphylos L. Fruit extract on serum lipids and hs-CRP levels and oxidative stress in adult patients with hyperlipidemia: a randomized, double-blind, placebo-controlled clinical trial. Evidence-Based complementary and alternative medicine.

https://doi.org/10.1155/2014/217451

Soltani, R., M. Hashemi, A. Farazmand, G. Asghari, K. Heshmat‐Ghahdarijani, A. Kharazmkia and S. M. Ghanadian, 2017. Evaluation of the effects of Cucumis sativus seed extract on serum lipids in adult hyperlipidemic patients: A randomized double‐blind placebo‐controlled clinical trial. Journal of food science, 82(1): 214-218. https://doi.org/10.1111/1750-3841.13569

Sudha, S. S., R. Karthic, J. R. Naveen and J. Rengaramanujam, 2011. Anti hyperlipidemic activity of Spirulina platensis in Triton x-100 induced hyperlipidemic rats. Hygeia journal of drug and medicines, 3(2): 32-37.

Surya, S., R. Arun Kumar, B. Carla and C. Sunil, 2017. Antihyperlipidemic effect of Ficus dalhousiae miq. stem bark on Triton WR-1339 and high fat diet-induced hyperlipidemic rats. Bulletin of faculty of pharmacy, Cairo university, 55: 73-77.

https://doi.org/10.1016/j.bfopcu.2016.10.003

Trease, E. G and W. C. Evan, 2007. Text Book of Pharmacognosy. Oxford: Alden Press.

United Nations. 2011. Globally Harmonized System of Classification and Labelling of Chemicals (GHS). 4th edition: United Nations. New York and Geneva.

Vetriselvan, S., U. Subasini, C. Velmurugan, T. Muthuramu and J. Revathy, 2013. Anti-inflammatory activity of Cucumis sativus seed in carrageenan and xylene induced edema model using albino wistar rats. International journal of biopharmaceutics, 4: 34-37.

Vogel, C., 2007. Medicinal plants in tropical countries. traditional use experience facts by Markus S. Mueller, MD (German Institute for Medical Mission) and Ernst Mechler, Ph. D. (University of Tuebingen). Thieme, New York. 2005. Journal of natural product, 70: 1068–1068. https://doi.org/10.1021/np0781458




DOI: https://doi.org/10.33865/wjb.007.03.0618

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Copyright (c) 2022 Tayyaba Aftab, Atiq ur Rehman, Abdullah Abdo Albegali, Amir Rashid, Ahsan Anjum, Mayada Mohammed, Hira Javed

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