Insilico analysis of peptides isolated from Agaricus bisporus manifests potential antimicrobial therapeutic activities

Aiman Fatima, Fatima Haider, Jaweria Malik Aftab Qaiser, Sami Ullah Jan, Syeda Marriam Bakhtiar


More and more microorganisms are progressively acquiring resistances to conventional antibiotics. Consequently, new antibiotics which are more effective are needed. Antimicrobial peptides (AMPs) are recognized as effective alternative to conventional antibiotics. The AMPs are obtained from different organisms including animals, plants, fungi, algae, and microorganisms. One of such significant sources of AMPs is Agaricus bisporus (button mushroom) that is long-familiar for its medicinal values. However, the occurrence and potential of AMPs in A. bisporus have not been well characterized till date. This study was aimed to identify AMPs within A. bisporus proteome and to further evaluate its antimicrobial potentials through in silico analysis. The proteome of A. bisporus was explored for antimicrobial peptides and their physicochemical properties were evaluated using bioinformatics tools. The proteome of A. bisporus contains 63 AMPs with ample antimicrobial properties such as broad spectrum efficacy, stable, non-allergenic and non-haemolytic attributes. It was further identified that these AMPs putatively target pathogens via membrane disruption and inhibition of ATP-dependent enzymes. This study renders a basis for further evaluation of identified AMPs through in vitro experimentations and trials to elucidate their practical use as therapeutic antimicrobial drugs. Resultantly, positive AMPs could be subjected to commercialization as cheaper and effective alternatives to conventional antibiotics


Antimicrobial, antibiotic resistance, button mushrooms, Agaricus bisporus, antimicrobial peptides, AMPs, drug resistance, multiple drug resistance, MDR

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