Insecticidal activity of essential oils of Cupressus arizonica greene and C. sempervirens L. on Tortrix viridana (Lepidotera, Tortricidae)

Yaussra Mannai, olfa Ezzine, Samir Dhahri, Mohamed Lahbib Ben Jama, Lamia Hamrouni


Tortrix viridana is one of the most important defoliators of oak trees. In 2006 and 2007, this insect caused significant defoliations of oak forests in the Northwestern Tunisia. This work aims at studying the insecticidal activity of essential oils of Cupressus arizonica and C. sempervirens on 3rd, 4th and 5th instars larvae of T. viridana by the contact test. Three different concentrations of essential oils (0.05%, 0.1% and 0.5%) were used to test their contact effectiveness; the Deltamethrin and the ethanol were used as positive and negative controls. Larvicidal activity was determined by measuring the Mean Mortality Time (MMT) to kill 100% of larvae. A total of 28 chemical compounds have been identified from C. arizonica and 20 chemical compounds from C. sempervirens. The difference in MMT of the 3rd instar larva was highly significant between the 3 concentrations of the two tested essential oils, the negative and positive controls. In fact, the concentration (0.5%) was more efficient than the two other concentrations with a MMT=1h31min12s for C. arizonica and 5h11min20s for C. sempervirens. The same results were observed for the 4th (MMT=3h23min24s (Ca) and 4h1min (Cs)) and 5th (MMT= 3h23min24s (Ca) and 4h01min (Cs)) instars. The results revealed that the essential oil of C. arizonica was more effective than C. sempervirens. Therefore, it is recommended to use the essential oil of C. arizonica and not that of C. sempervirens, given its insecticidal efficiency on the 3rd instar larva of T. viridana


Tortrix viridana; Cupressus arizonica; C. sempervirens; larvicidal activity

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Afsharypuor, S. and P. Tavakoli, 2005. Essential oil constituents of leaves and fruits of Cupressus arizonica greene. Journal of essential oil research, 17(2): 225-226.

Akkari, H., O. Ezzine, S. Dhahri, F. B’chir, M. Rekik, S. Hajaji, M. A. Darghouth, M. L. B. Jamâa and M. Gharbi, 2015. Chemical composition, insecticidal and in vitro anthelmintic activities of Ruta chalepensis (Rutaceae) essential oil. Industrial crops products, 74: 745-751.

Al-Mouhajer, L., R. Chabo, A. M. Saab, K. Saade and H. Makhlouf, 2017. Antibacterial activities of essential oils isolated from two species Cupressus arizonica greene and Cupressus sempervirens L. (Var. Horizontalis and pyramidalis). European journal of biomedical, 4(12): 430-435.

Ali, A., N. Tabanca, B. Demirci, K. H. C. Baser, J. Ellis, S. Gray, B. R. Lackey, C. Murphy, I. A. Khan and D. E. Wedge, 2013. Composition, mosquito larvicidal, biting deterrent and antifungal activity of essential oils of different plant parts of Cupressus arizonica var. Glabra (‘Carolina sapphire’). Natural product communications, 8(2): 1934578X1300800232.

Amri, I., L. Hamrouni, M. Hanana, S. Gargouri, T. Fezzani and B. Jamoussi, 2013. Chemical composition, physico-chemical properties, antifungal and herbicidal activities of pinus halepensis miller essential oils. Biological agriculture horticulture, 29(2): 91-106.

Boukhris, M., G. Regane, T. Yangui, S. Sayadi and M. Bouaziz, 2012. Chemical composition and biological potential of essential oil from tunisian Cupressus sempervirens L. Journal of arid land studies, 22(1): 329-332.

Chaudhary, H. J., W. Shahid, A. Bano, F. Ullah, F. Munis, S. Fahad and I. Ahmad, 2012. In vitro analysis of Cupressus sempervirens L. Plant extracts antibaterial activity. Journal of medicinal plants research, 6(2): 273-276.

Donato, R., C. Sacco, G. Pini and A. R. Bilia, 2020. Antifungal activity of different essential oils against malassezia pathogenic species. Journal of ethnopharmacology, 249: 112376.

Elansary, H. O., M. Z. Salem, N. A. Ashmawy and M. M. Yacout, 2012. Chemical composition, antibacterial and antioxidant activities of leaves essential oils from Syzygium cumini L., Cupressus sempervirens L. and Lantana camara L. From Egypt. Journal of agricultural science, 4(10): 144.

Enan, E., 2001. Insecticidal activity of essential oils: Octopaminergic sites of action. Comparative biochemistry physiology part C: toxicology pharmacology, 130(3): 325-337.

Ezzine, O., M. Branco, C. Villemant, S. Schmidt, S. Nouira and M. L. B. Jamâa, 2015. Host use in Orgyia trigotephras (Erebidae, lymantriinae) during outbreak: Effects on larval performance and egg predation. Annals of forest science, 72(5): 561-568.

Mannai, Y., M. Ben Jamâa, S. M'nara, H. Selmi and S. J. Nouira, 2010. Biology of Tortrix viridana (Lep., Tortricidae) in cork oak forests of North-west Tunisia. 57: 153-160.

Mannai, Y., O. Ezzine, A. Hausmann and M. Jamâa, 2018. First report of three tortricidae species on quercus suber forest in Northwestern Tunisia. Tunisian journal of plant protection, 13(2): 275-280.

Oroojalian, F., R. Kasra-Kermanshahi, M. Azizi and M. R. Bassami, 2010. Phytochemical composition of the essential oils from three apiaceae species and their antibacterial effects on food-borne pathogens. Food chemistry, 120(3): 765-770.

Rawat, P., M. F. Khan, M. Kumar, A. K. Tamarkar, A. K. Srivastava, K. R. Arya and R. Maurya, 2010. Constituents from fruits of cupressus sempervirens. Fitoterapia, 81(3): 162-166.

Rguez, S., N. Djébali, I. B. Slimene, G. Abid, M. Hammemi, S. Chenenaoui, S. Bachkouel, M. Daami-Remadi, R. Ksouri and I. Hamrouni-Sellami, 2018. Cupressus sempervirens essential oils and their major compounds successfully control postharvest grey mould disease of tomato. Industrial crops products, 123: 135-141.

Riahi, L., M. Elferchichi, H. Ghazghazi, J. Jebali, S. Ziadi, C. Aouadhi, H. Chograni, Y. Zaouali, N. Zoghlami and A. Mliki, 2013. Phytochemistry, antioxidant and antimicrobial activities of the essential oils of Mentha rotundifolia l. In tunisia. Industrial crops products, 49: 883-889.

Ruiu, L., A. Lentini, M. Coinu, A. Loi, G. Serra and P. Luciano, 2012. Comparative applications of Bacillus thuringiensis formulations against Lymantria dispar in sardinian forests. IOBC/wprs Bull, 76: 185-190.

Sedaghat, M. M., A. S. Dehkordi, M. Khanavi, M. R. Abai, F. Mohtarami and H. Vatandoost, 2011. Chemical composition and larvicidal activity of essential oil of Cupressus arizonica el greene against malaria vector Anopheles stephensi liston (Diptera: Culicidae). Pharmacognosy research, 3(2): 135.

Slimane, B. B., O. Ezzine, S. Dhahri and M. L. B. Jamaa, 2014. Essential oils from two eucalyptus from Tunisia and their insecticidal action on Orgyia trigotephras (Lepidotera, lymantriidae). Biological research, 47(1): 1-8.

Tiberi, R., D. Benassai and A. Niccoli, 2005. Influence of different host plants on the biology and behaviour of the green oak leaf roller, Tortrix viridana L.: First results. IOBC WPRS Bulletin, 28(8): 211.


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